aedes larvae identification

Entomol. (2014). 11:359. doi: 10.1186/s13071-018-2883-8. 88, 62946306. Likewise, a longer sampling time that could allow us a greater capture of individuals and a more general view of the behavior of the infection of arboviruses in A. aegypti and A. albopictus. J. Mosquito-borne diseases: advances in modelling climate-change impacts. Nevertheless, the molecular biology approach for mosquito identification remains laborious, time-consuming and costly [18]. (2015). Dis. DENV-1 was the arbovirus with the highest frequency in the population of infected individuals at 82% (50/61) and the overall infection rate in A. aegypti and A. albopictus was 29.6% (50/169) (Table 2). (2019) showed that DENV-1 strains isolated in Venezuela were closely related to previously published sequences of this serotype in Colombia. However, occasionally, exuviae collected directly from the field could not yield sufficient genomic DNA for amplification [38]. (2020). This agrees with the results obtained in this study, given that DENV-1 is the most prevalent arbovirus in the department of Arauca (Boletn Epidemiolgico Semanal (BES) Semana Epidemiolgica 22, 24 al 30 de mayo, 2020). The DENV-1 serotype has also been detected more frequently than other arboviruses in Indonesia, Mexico, Cuba, Brazil, and Colombia (Velandia-Romero et al., 2017; Eiras et al., 2018; Garcia-Rejon et al., 2018; Gutirrez-Bugallo et al., 2018; Rahayu et al., 2019). Furthermore, the global diversity of haplotypes for all individuals captured in Arauca was h = 0.639. The protocol recommended by the WHO (World Health Organization [WHO], 2020) was used for the MAYV detection. Chikungunya virus in US travelers returning from India, 2006. MM carried out the statistical and phylogenetic analysis of the results obtained.

doi: 10.2987/09-5945.1, Hou, V., Bonizzoni, M., and Failloux, A. Longitudinal monitoring of environmental factors at Culicidae larval habitats in urban areas and their association with various mosquito species using an innovative strategy. Among them, the expression of some cuticular proteins was restricted to a single metamorphic stage, which could explain the singularity of MS profiles between exuviae from the same species. albopictus and Ae.

The amplification of the gene fragment was conducted using 2.5% agarose gel electrophoresis in TBE 1x buffer and SYBR Safe (Invitrogen, Carlsbad, CA, United States) as the intercalating agent and then visualized under UV light to observe a band of 650 base pairs (bp). Kappa. doi: 10.1007/s10393-019-01417-3. Detection of invasive mosquito vectors using environmental DNA (eDNA) from water samples.

(2019). Received: 02 September 2020; Accepted: 04 November 2020;Published: 26 November 2020. Health 12:853. doi: 10.1186/1471-2458-12-853, Shope, R. E., and Meegan, J. M. (1997). Analytical and clinical performance of the CDC real time RT-PCR assay for detection and typing of dengue virus. Bonizzoni M, Gasperi G, Chen X, James AA. The reproducibility of MS spectra was confirmed by CCI matrix highlighting the good correlation between exuviae of the same species and stage (meanSD: 0.800.14; Fig. doi: 10.1093/cid/cis530, Cevallos, V., Ponce, P., Waggoner, J. J., Pinsky, B. Analyzing the spatio-temporal relationship between dengue vector larval density and land-use using factor analysis and spatial ring mapping. Barnes MA, Turner CR, Jerde CL, Renshaw MA, Chadderton WL, Lodge DM.

Infect. (2019) for individuals captured in Colombia and Joyce et al. Med. Trans. This strategy will overcome the limitations of morphological and molecular identification and will avoid the use of the entire body of the specimen, as it was done previously [22]. 14:726736 doi: 10.4257/oeco.2010.1403.08, Garcia-Rejon, J. E., Ulloa-Garcia, A., Cigarroa-Toledo, N., Pech-May, A., Machain-Williams, C., Cetina-Trejo, R. C., et al. The selected peaks by the operator gave a recognition capability (RC) value together with the highest cross-validation (CV) value. 93.8% (135/144) of exuviae LSVs reached the threshold value (LSVs>1.8) for reliable identification [24, 27], among which 100% of the samples were correctly classified at the species and stage levels. 11 Articles, This article is part of the Research Topic, Boletn Epidemiolgico Semanal (BES) Semana Epidemiolgica 22, 24 al 30 de mayo, 2020,,, Creative Commons Attribution License (CC BY). Google Scholar. Asymptomatic humans transmit dengue virus to mosquitoes. 2014;113:23758. (2019). Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Mem.

LA conceived and designed the experiments, performed the experiments and analyzed the data. Parasit Vectors. EcoHealth 16, 210221. Mayaro virus distribution in South America. Additionally, due to its close proximity to Venezuela, attempting to identify MAYV is worthwhile (Lorenz et al., 2019). Vogels CB, Goertz GP, Pijlman GP, Koenraadt CJ. Potential effects of heat waves on the population dynamics of the dengue mosquito Aedes albopictus. In addition, the infection by DENV-1, CHIKV, ZIKV, and MAYV was attempted. Abbreviations: A.U., arbitrary units. b The same four MS spectra per species and developmental stages were analysed using the composite correlation index (CCI) tool. doi: 10.1093/jmedent/29.1.49, Vega-Ra, A., Zouache, K., Girod, R., Failloux, A.

To face of this growing vulnerability of European countries to MBDs, the implementation of guidelines for surveillance and control of invasive mosquito was established [15]. Int. Failure in DNA amplification has been attributed to the decomposition of epithelial cells attached to the exuviae [38]. 2015;12:120. Am J Trop Med Hyg. At the beginning of the pupal cycle, MS profiles are shared with counter species MS spectra from late larval instar stages explaining specimen identification success [22]. mosquito larvae biology larva mosquitoes water pupa egg data stages insect adult aedes control larval dengue da river aegypti ciclo Freitas, R. M. D. (2010). Future studies should consider evaluating the transmission dynamics of arboviruses in this region by examining the plausible biological competition of the two species. According to the kappa index obtained in this study between the identification methods (k = 0.7643) (Cook, 2005), a deficiency in identification through morphology can occur (Figure 2). For the results of the geographic distribution of individuals and their natural infection by arboviruses, molecular identification of the species was considered. The qRT-PCR reactions for DENV-1, CHIKV, and ZIKV (per sample) were performed in a final volume of 20 L containing 10 L qScriptTM One-Step, Low ROXTM (Quantabio, Carlsbad, CA, United States), 10 M of each primer, 10 M of the probe and 5 L of RNA. albopictus and Ae. CCI were expressed as the meanstandard deviation. Mosquitos (Culicinos) de Venezuela: Tomo I and Tomo II. Evol. Municipalities where captures were conducted: Saravena (green), Arauquita (pink), and Tame (blue) in the department of Arauca (purple), Colombia. Cova-Garcia, P., Sutil, E., and Rausseo, J. Due to these difficulties, different authors mention that the identification of insects based on deoxyribonucleic acid (DNA) can become a more efficient technique when classifying individuals (Rolo, 2020). Accurate identification of Culicidae at aquatic developmental stages by MALDI-TOF MS profiling. Parasit Vectors. Faculty of Public Health, University of So Paulo, Brazil. In the field, exuviae generally degrade quickly.

were obtained for more than 95% (153/160) of exuviae from the two Aedes species. Parasites & Vectors

Additionally, the genus Culex (included as an outgroup) formed an independent clade in the species of genus Aedes. Two samplings were conducted, one in 2018 and the other in 2019, and each BG-Mosquitaire was installed for three consecutive months during the rainy season (AprilMayJune). mosquito mosquitoes aedes aegypti Res. (2018) for El Salvador; these values are relatively high. Reliable species identification is indispensable for adapting mosquito vector control strategies. MALDI-TOF MS biotyping emerged recently as a relevant alternative solution, to dispense with morphological and molecular biology identification tools [18]. The mosquito exuviae correspond to shedding of the exoskeleton and related structures. doi: 10.1016/j.actatropica.2017.09.029, Chan, A., Chiang, L. P., Hapuarachchi, H. C., Tan, C. H., Pang, S. C., Lee, R., et al. mosquito bug bugs flies database Res. doi: 10.7705/biomedica.v37i0.3584, Villar, L. A., Rojas, D. P., Besada-Lombana, S., and Sarti, E. (2015). J. Infect. In A. aegypti, DENV presents a higher range of MIR compared to other viruses and ranges from 0.2 to 26 infected individuals per 1,000 individuals. Boletn Epidemiolgico Semanal (BES) Semana Epidemiolgica 22, 24 al 30 de mayo (2020). (1980). software was also used, to assess the spectral variations within and between sample groups, as previously described [20, 26]. Mosq Syst.

MEGA biocentric software for sequence and phylogenetic analysis: a review. Modelling dengue epidemic spreading with human mobility. *Correspondence: Juan David Ramrez,, Biology, Systematics, Taxonomy, and Evolution of Insect Vectors, View all Entomol. Parasit Vectors. Development of guidelines for the surveillance of invasive mosquitoes in Europe. CAS Moreover, the euthanasia of whole larvae hinders assess to other mosquito parameters such as insecticide susceptibility or vector competence. Euro Surveill. The insects were captured in the department of Arauca in the municipalities of Saravena (6 5717 N, 71 5236 W), Arauquita (7 0134 N, 71 2538 W), and Tame (6 2730 N, 71 4441 W) (Figure 1). 9:e0004296. (2005). The black dot indicates the urban area where the capture of individuals was made. Monitoring of alien mosquitoes of the genus Aedes (Diptera: Culicidae) in Austria. Ecol.

This confirms that this arbovirus is not circulating in the urban cycle of the three sampled municipalities. J. Environ. 1994;26:1924. CAS fourth-instar larvae and pupae) were used to construct the MSP dendrogram. The raw data of exuviae MS spectra from Ae. Hygiene 111, 328331. 2015;71:147185. The reliability of species identification was estimated using the log score values (LSVs) obtained from the MALDI Biotyper software v.3.0, which ranged from 0 to 3. Ongoing outbreak of dengue type 1 in the Autonomous Region of Madeira, Portugal: preliminary report. By keeping the specimen intact, complementary analysis on the same specimen is possible, such as insecticide susceptibility or vector competence. Therefore, it is important that future studies implement viral enrichment techniques that allow virus detection even at low concentrations. MS spectra reproducibility and specificity were objectified using cluster analyses and composite correlation index (CCI) tool. Lee H, Halverson S, Ezinwa N. Mosquito-borne diseases. Med. By using this website, you agree to our software with clustering according to protein mass profile (i.e. LA and AN drafted the paper and critically revised the paper.

The setting parameters of the MALDI-TOF MS apparatus were identical to those previously used [25]. Int.

Prevalence and distribution of dengue virus in Aedes aegypti in yogyakarta city before deployment of Wolbachia infected Aedes aegypti. Aedes aegypti is also present in Europe and was responsible for a dengue outbreak in the region of Madeira which occurred in October 2012 [7]. We would like to thank the Direccin de Investigacin e Innovacin from Universidad del Rosario for funding this study and providing the editing English service of ENAGO and cover the publication fees. This shows a simultaneous circulation of these two arboviruses within one specimen, which can lead to coinfection in humans, where an increase in coinfection cases would lead to rapid genetic evolution of the virus (Caron et al., 2012). Moreover, global warming facilitated the colonization of new areas by mosquitoes participating to the rapid change of the mosquito-borne diseases (MBDs) epidemiology [3, 4]. One MS peak was predominant in fourth-instar exuviae from Ae. Larval development of Aedes aegypti and Aedes albopictus in peri-urban brackish water and its implications for transmission of arboviral diseases. Furthermore, no CHIKV and MAYV infection has been detected in the latter. 2. B. In this study, a mixed DENV-1 and CHIKV coinfection rate of 4.5% (5/111) was found in A. aegypti. Exuviae from fourth-instar and pupae were homogenized individually 31 min at 30 Hertz using a TissueLyser (Qiagen, Hilden, Germany) and glass beads of 1.0 mm diameter (product No.
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